Composite

Part:BBa_K1918101

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)


TRE-Kozak-EBFP2N(1-154)

This part is consist of EBFP2N(1-154) and the TRE promoter. EBFP2N(1-154) is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of EBFP2N(1-154) could be induced by Dox.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]


Template:Tsinghua-A

    In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.

    Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.

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Categories
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